Using biosensors and light probes to investigate biological signalling in 3D models (spheroids, organoids, tissues and multicellular assemblies)

Program:

9:30 Welcome  : introduction to GDR Imabio, and to sponsors

9:45 Session 1: Light probes for normal and pathological development

-  Opening keynote : Vanessa Ribes (Institut Jacques Monod, Paris) Modelling and imaging of morphogenesis defects associated with spina bifida using iPSC-derived organoids

Short talks :

• Maxime Mauviel Development of an opto-microfluidic assay, to probe signaling and function in glomeruli-on-chip.
• Gabriel Bidaux High-sensitivity calcium biosensor on the mitochondrial surface reveals that IP3R channels participate in the reticular Ca2+ leak towards mitochondria

Sponsors’ short talk

11:00 Coffee break

11:30 Session 2: Organoids to understand and treat diseases

- Keynote : Olivier Goureau (Institut de la Vision, Paris) Stem cell-based retinal organoids: Structural and functional analysis to understand human retinal pathophysiology

- Short talks :

• Corinne Lebreton iPSC-Derived Intestinal Organoids for for the modelling of epithelial polarity defects
• Alessandro Furlan Tumor-on-chip models to recapitulate the tumor microenvironment

Lunch and poster session 13:00

14:00 Session 3: Biophysics unraveled thanks to light

-  Keynote : Michael Krieg (The Institute of Photonic Sciences ICFO, Barcelona) Shining a light on mechanical body-brain interactions during touch and proprioception

- Short talks :

• Fanny Wodrascka Role of mechanical forces in cell extrusions and cell fate using optogenetics
• Valentin Dunsing Quantitative 3D live-imaging of self-organisation in embryonic organoids

-  Keynote : Thomas Boudou (Laboratory of Interdisciplinary Physics (LIPhy), Grenoble) Engineering and actuating 3D microtissues

Sponsors’ short talk

16:15 Coffee break

16:45 Session 4: FRET biosensors to monitor and quantify molecular events

Short talks :

-  Louis Laurent Control of ERK activity by epithelial density

-  Marcelina Cardoso Dos Santos Development of Förster Resonance Energy Transfer nanosensors for the study of molecular assembly

-  Ending keynote: Bas Ponsioen (Hubrecht Institute, Utrecht) Real-time single-cell monitoring of ERK activity in colorectal cancer organoids : from short-term to long-term analyses

Venue:

Institut Jacques Monod, Paris (see MAP tabs for further details)

Organising committee:

Gabriel Bidaud

Frédéric Bolze

Nicolas Borghi

Lydia Danglot

Alessandro Furlan

Philippe Girard

Corinne Lebreton

Véronique Proux

Gaëlle Recher

Franck Riquet

François Siepeter